In-vitro evaluation of antimicrobial activity of Dhupana drugs on Staphylococcus aureus

Abstract

Multidrug resistance in the notorious bacterial strains is accounting for a tremendous increase in global mortality rate. The upcoming new generation antibiotics are granting a perceptive provision in this issue but the superbugs have enforced these antimicrobial agents to surrender by developing the innovative variation in their genes/ plasmids. So alternatives are desired to combat this problem. Herbal drugs may broaden this spectrum of antimicrobial agents by offering safer and promising approach. Dhupana karma is such a modality known for its antimicrobial activity due to its indication in infectious diseases as per classical texts. The present study included fumigation on Staphyloccus aureus with four Dhupana formulations to assess their antimicrobial activity. The assessment criteria taken was mean bacterial colony count after 20 min of fumigation compared with control (without fumigation). Statistical analysis was done using Anova test and Tukey Kramer comparison test. The % change in mean colony count of B1 (S.aureus) after 20 min of fumigation was found to be 76.61 % with drug D1 (Nimbadi Dhupa), 84.13 % with drug D2 (Kumaragara Dhupa), 80.51 % with drug D3 (Dashanga Dhupa) and 98.94 % with drug D4 (Gana Dhupa).