Evaluation of Growth Inhibitory Activities of Extracts of Whole Plant of Emblica officinalis Gaertn. on Gram-Positive and Gram-Negative Bacteria
Keywords:
Emblica officinalis, antibacterial assay, well diffusion, aqueous extract, S. aureus, E. coliAbstract
The present study focussed on the quantitative estimation of in vitro antibacterial activity of aqueous extracts of fruits, stem, root, leaves and seeds of Emblica officinalis against Gram-positive versus Gram-negative bacteria. The study employed Staphylococcus aureus and Escherichia coli, respectively, as the test organisms and the assay was carried out by using agar well diffusion method. S. aureus was more susceptible to the extracts of fruits, stem, leaves and seeds of Emblica. The aqueous extract of leaves maximally inhibited the growth of S. aureus at its minimum concentration (0.1 µg) when compared to E. coli (1.0 µg). MIC for fruit and seed extracts were 1.5 and 3.75 µg and 1.5 and 5.0 µg, respectively, for S. aureus and E. coli. The aqueous extract of stem had the least activity where more quantities of the extract were required to inhibit the growth of both S. aureus (15.0 µg) and E. coli (20.0 µg). Both the test organisms were resistant to the aqueous extracts of root of Emblica officinalis. It could be attributed from the present study that the inhibitory effect of Emblica officinalis is more against Gram-positive than Gram-negative bacteria. The leaf of Emblica is more inhibitory to the growth of bacteria than fruits, seeds or stemDownloads
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