Qualitative and quantitative screening of phytoconstituents and characterization of various extracts of Wedelia trilobata leaf extracts

Abstract

The birth control pills (contraceptive) are safe, in some cases the combination of the contraceptive pill can increase your risk of health. The side effects include heart attack, stroke, blood clots, and liver tumors. In very rare cases, they can lead to death. The risk of health problems and side effects were minimal or no side effects recorded while using the herbal contraceptives. The present investigation was focused on the preliminary phytochemical analysis, UV-VIS spectrum and Fourier Transform Infrared Spectral analysis and HPLC analysis of Wedelia trilobata L. The leaves were extracted using soxhlet extractor with seven different solvent in the order of relative polarity (aqueous, methanol, ethanol, acetone, petroleum ether, benzene and hexane) the leaves of the plant of Wedelia trilobata L.  were tested for the availability of alkaloids, glycosides, flavonoids, phenols, saponins, steroids, amino acids, tannins, terpenoids, quinones, anthraquinones etc. And the phenol, flavonoid and terpenoids were analysed quantitatively. The UV-VIS spectrum showed the peaks with the absorption for the seven solvents respectively. The FT-IR spectrum showed the presence of alcohols, phenols, alkanes, alkynes, alkyl halides, aldehydes, aromatics, nitro compounds and amines. With the HPLC results we confirm that the compound caryophyllene which was used as contraceptive now a days was present in the two extracts (aqueous and ethanol) The results confirm the fact that this plant possesses important bioactive constituents useful for our health, so further scientific investigation is needed to investigate the biological activities of this plant.