Formulation and evaluation of antifungal activity of Kasisadi Varti against Candida albicans

Abstract

Background: Medicine is said to be of good quality when it gives the desired therapeutic effect and at the same time does not cause any harm to the patients. The safety and efficacy of a medicine is of at most importance. Preclinical studies play a vital role in ascertaining the safety and efficacy of a drug before taking it for clinical trial. They mainly involve invitro and invivo models. Kasisadi churna is a formulation mentioned for external application in kaphaja yoni vyapath. Application of churna (powder) externally may not be compatible on patients, and hence the dosage form was modified into varti (suppository) form. Objective: Invitro study to asses antifungal activity of kasisadi varti. Material and Methods: Kasisadi varti were prepared in three different methods. They were assessed for activity against Candida albicans by invitro Agar well diffusion method at different concentrations 32mg/dl, 64mg/dl, 128mg/dl, 256mg/dl and 512 mg/dl. Result: All three samples showed significant activity against Candida albicans at a concentration of 128 mg/dl, 256mg/dl and 512mg/dl. Conclusion:All three samples showed significant results on Candida albicans developed from vaginal smears of patients at a concentration of 128mg/ml, 256mg/ml, and 512 mg/ml in comparison with standard drug Amphotericin B. None of the samples showed any effect on MTCC Strain of Candida albicans.