Study of physicochemical properties of Indrayava (Holarrhena antidysenterica wall.) and its antibacterial effect on Enteropathogenic e-coli (EPEC) (in vitro)

Abstract

As stated in Ayurvedic texts Indrayava mainly useful in treatment of diseases like diarrhoea, dysentery etc. E-coli is most dangerous bacteria causing diarrhoea. Ayurveda has included all the microbes under the heading 'Krimi'. As per Bhavaprakasha Nighantu, Indrayava having Atisaraghna and Krimighna property. Hence Indrayava having action on bacteria (Krimi) may have action on E.coli causing diarrhoea (EPEC). So it is necessary to do the physicochemical standardization of Indrayava, to study its antibacterial activity on EPEC (In-vitro), to determine minimum inhibitory concentration of Indrayava for antibacterial activity against EPEC. Materials used are self collected sample, clinically isolated EPEC. Method used for antibacterial susceptibility is disc diffusion method. After study result came are, foreign matter is negligible, moisture content is 8.57%, total ash is 4.61%, acid insoluble ash is 0.60%, water soluble ash is 6.67%, water soluble extract is 33.32%, alcohol soluble extract is 30.51% and pH is 5.42. Indrayava shows the antibacterial activity against Enteropathogenic E-coli (EPEC) in methanolic extract having minimum inhibitory concentration value 2.0gm/10ml.