Phytochemical analysis of Clerodendron infortunatum Linn. leaves in various seasons by different solvent and extraction techniques

Abstract

Clerodendron infortunatum Linn is widely used for the treatment of malaria and vermifuge, scorpion and snake bites, tumors, inflammation, bronchitis and in wound healing. The present study was carried out to estimate the variation in the amounts of Gallic acid, Rutin and Quercetin in Clerodendron infortunatum Linn.leaves extracts collected in different seasons using high-performance thin layer chromatography (HPTLC).In this study, leaves of C. infortunatum were subjected to methanol extractions using Soxhlet method and cold maceration. The leaves were collected in different seasons in the months of December (Sample A -Shishir rutu-Winter season) and July (Sample B -Varsha Rutu -Rainy season). The methanolic extracts were subjected to HPTLC study & using CAmag HPTLC system, employing a mixture of toluene:ethyl acetate:formic acid:methanol(3:6:1.6:0.4 ) as the mobile phase with densitometric scanning at 254nm. Quantity of Quercetin is found in both samples, extracted by Soxhlet method. Its quantity is higher in the winter season. Quantity of Gallic acidis found in both samples. Its quantity is the same in Sample B by Soxhlet extraction method & maceration method respectively. Quantity of Rutin is found in both the samples extracted by Soxhlet & maceration methods. It is higher in the sample-A. The present HPTLC method is used for the detection and quantization of Gallic acid, Quercetin and Rutin of C. infortunatum leaves. It needs to study the season wise and region wise variation of phytochemicals in useful parts of all other plants by different extraction methods.