Simultaneous method development and validation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC

Authors

  • Krupa Joshi
  • Jaya Patel
  • Swapnil Raskar
  • Chandani Aghara

DOI:

https://doi.org/10.47552/ijam.v13i3.2990

Keywords:

HPTLC, 1-8 Cineole, Betulinic acid, Shati vati an Ayurvedic formulation, ICH Q2 (R1) guideline, Method validation

Abstract

A simple, accurate and precise HPTLC method was developed and validated for simultaneous estimation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC. Shati Vati contains Shati rhizome powder have 1-8 cineole and Badar ripe fruit powder have Betulinic acid reported medicinal claims and uses.  In the analytical method, toluene: ethyl acetate (93:07 v/v) was finalized as the mobile phase and a clear separation was achieved. The wavelength for 1-8 cineole and Betulinic acid was 665nm and 580nm respectively. The Rf value of 1-8 cineole and betulinic acid was obtained 0.647 and 0.213 respectively. The developed analytical method was validated for Linearity, Accuracy, Precision, LOD, LOQ and Robustness as per ICH Q2 (R1) guideline. Results for the validation process were within the range of acceptance criteria of ICH guideline.  HPTLC method have been applied successfully for estimation of 1-8 Cineole and Betulinic acid in Shati Vati an ayurvedic formulation.

Author Biographies

Krupa Joshi

Student, Parul Institute of Pharmacy and Research, Parul University. Vadodara. India.

Jaya Patel

Assistant Professor, Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University. Vadodara. India.

Swapnil Raskar

 Assistant Professor,  Faculty of Ayurveda, Parul Institute of Ayurveda, Parul University. Vadodara. India. 

Chandani Aghara

PG Scholar, Faculty of Ayurveda, Parul Institute of Ayurveda, Parul University. Vadodara. India.

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Published

09-10-2022

How to Cite

Joshi, K., Patel, J., Raskar, S. ., & Aghara, C. . (2022). Simultaneous method development and validation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC. International Journal of Ayurvedic Medicine, 13(3), 625–628. https://doi.org/10.47552/ijam.v13i3.2990

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Section

Research Articles