Simultaneous method development and validation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC
Keywords:HPTLC, 1-8 Cineole, Betulinic acid, Shati vati an Ayurvedic formulation, ICH Q2 (R1) guideline, Method validation
A simple, accurate and precise HPTLC method was developed and validated for simultaneous estimation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC. Shati Vati contains Shati rhizome powder have 1-8 cineole and Badar ripe fruit powder have Betulinic acid reported medicinal claims and uses. In the analytical method, toluene: ethyl acetate (93:07 v/v) was finalized as the mobile phase and a clear separation was achieved. The wavelength for 1-8 cineole and Betulinic acid was 665nm and 580nm respectively. The Rf value of 1-8 cineole and betulinic acid was obtained 0.647 and 0.213 respectively. The developed analytical method was validated for Linearity, Accuracy, Precision, LOD, LOQ and Robustness as per ICH Q2 (R1) guideline. Results for the validation process were within the range of acceptance criteria of ICH guideline. HPTLC method have been applied successfully for estimation of 1-8 Cineole and Betulinic acid in Shati Vati an ayurvedic formulation.
How to Cite
Copyright (c) 2022 International Journal of Ayurvedic Medicine
This work is licensed under a Creative Commons Attribution 4.0 International License.
The author hereby transfers, assigns, or conveys all copyright ownership to the International Journal of Ayurvedic Medicine (IJAM). By this transfer, the article becomes the property of the IJAM and may not be published elsewhere without written permission from the IJAM.
This transfer of copyright also implies transfer of rights for printed, electronic, microfilm, and facsimile publication. No royalty or other monetary compensation will be received for transferring the copyright of the article to the IJAM.
The IJAM, in turn, grants each author the right to republish the article in any book for which he or she is the author or editor, without paying royalties to the IJAM, subject to the express conditions that (a) the author notify IJAM in advance in writing of this republication and (b) a credit line attributes the original publication to IJAM.