Determination of Total phenolic content and Antiurolithiatic activity of Sahasravatamulika (Hibiscus cannabinus Linn.) Extract and Fractions

Abstract

The purpose of this study was to assess the total phenol content and invitro antiurolithiatic activity of Hibiscus cannabinus extract and various fractions. Kidney stones are one of the most painful and prevalent urinary tract disorders. Drug treatment and Extracorporeal Shock Wave Lithotripsy (ESWL) are used to treat kidney stones. Instead of dissolving the stone, these treatments may cause acute renal damage and an increase in stone production. Phenolic compounds show the best stone dissolution rate, i.e., they could dissolve calcium oxalate stones. In the present research Hibiscus cannabinus phenolic fraction was tested for its capacity to dissolve calcium phosphate stones, which is one of the many forms of kidney stones that can form. The highest phenolic content was found in the Ethyl acetate fraction of the plant, which was assessed using Gallic acid as a standard. This fraction was then tested for Antiurolithiatic activity in an in-vitro manner employing the egg's semipermeable membrane. The fraction can be evaluated for additional in-vivo and in-vitro investigations to determine its ability to dissolve Kidney stones developed in humans.