In-vitro anti-inflammatory and anti-oxidant study of Sansevieria cylindrica Bojer ex. Hook and Plumeria obtusa L. plants using different methods

Abstract

Background: Sansevieria cylindrica and Plumeria obtusa are used as a medicinal plant during intentional and unintentional accidental injuries. However, limited investigations have been performed to study pharmacological activities of these plants. Objectives: The current study designed at examining in-vitro anti-inflammatory and anti-oxidant effect of both plant extracts. Material & Method: Initially, the collection and authentication of both the plants performed. Phytochemical screening was done thereafter. Sansevieria cylindrica leaves and Plumeria obtusa seed pods were extracted using a combination of water and ethanol. Anti-inflammatory effect was assessed using membrane stabilization and protein denaturation assays. Anti-oxidant activity was measured by free radicals scavenging method using different reactive oxygen species producing reagents. Results: The dose dependent increase in anti-inflammatory and anti-oxidant activities were reported by both plants. Overall, Sansevieria cylindrica has shown higher rate of prevention of inflammation and oxidation compared to Plumeria obtusa extract. Both plants showed comparable anti-inflammatory and anti-oxidant activity in combination with that of reference drugs. Conclusion: The hydro-alcoholic extracts of Sansevieria cylindrica and Plumeria obtusa individually and also as 1:1 blend might be responsible for an anti-inflammatory and anti-oxidant activities.