Development and Validation of UV-Spectrophotometric Method for Estimation of Vinpocetine in Marketed Formulation and Nanoformulation

Authors

  • Chethan Kumar HB
  • Bhaskar Kurangi
  • Moazzim Soudagar
  • Supriya Chimagave
  • Swapnil Patil

DOI:

https://doi.org/10.47552/ijam.v14i3.3585

Keywords:

UV- visible spectrophotometric method, Vinpocetine, Validation, Marketed formulations, Stability study, Periwinkle

Abstract

A quick, accurate, and cost-effective UV spectroscopy method was developed to estimate the Vinpocetine concentration in bulk, tablet dosage formulations and niosomes formulations, using a solvent ratio of (6:4) methanol: water. According to ICH guidelines, the proposed technique was validated and developed. In spite of linearity, precision, accuracy, specificity, LOD, and LOQ, like parameters were validated by using UV/visible spectroscopy technique to analyze a spiked Vinpocetine solution. The wavelength at which the drug's maximum absorbance peak was obtained at 274 nm and the solvents used as methanol: water (6:4 w/v). The ethanol injection technique was used to prepare niosomes to analyze Vinpocetine in UV / visible spectrophotometric method.During the inter and intra-day studies, it was discovered that the developed UV technique was accurate, with % relative standard deviation ranging from 0.27 to 0.46 and 0.26 to 0.46, respectively. Vinpocetine overall recovery percentage was discovered to be between 98.42 to 99.82 %. LOQ and LOD were calculated to estimate the method's sensitivity, and they were observed to be 0.4565 µg/ml and 0.1506 µg/ml, respectively. The estimation of Vinpocetine content in bulk form, marketed formulations and niosomes was achieved using the developed methodology. : A quick, accurate, and economical UV spectrophotometric method has been developed. As a result, the suggested UV spectroscopic technique has been developed to estimate the vinpocetine concentration in bulk, tablet dosage formulations, and niosomes formulations.

Author Biographies

Chethan Kumar HB

Department of Pharmaceutics, KLE College of Pharmacy, KLE Academy of Higher Education and Research, Belagavi. India. 

Bhaskar Kurangi

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India.

Moazzim Soudagar

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India.

Supriya Chimagave

Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India.

Swapnil Patil

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India.

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Published

03-10-2023

How to Cite

Kumar HB, C. ., Kurangi, B., Soudagar, M., Chimagave, S. ., & Patil, S. . (2023). Development and Validation of UV-Spectrophotometric Method for Estimation of Vinpocetine in Marketed Formulation and Nanoformulation. International Journal of Ayurvedic Medicine, 14(3), 717–723. https://doi.org/10.47552/ijam.v14i3.3585

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Section

Research Articles