Development and Validation of Analytical Method for Estimation of Berberine in Herbal Extract of Momordica Dioica
Keywords:Berberine, HPTLC, Herbal Extract, Momordica Dioica
Background:While several anti-diabetic medications have been found to lower blood glucose levels, there are numerous negative effects connected with the use of currently marketed anti-diabetic medications.However, there are medicinal plants with anti-diabetic effects with low or no side effects. Among the Cucurbitaceae family of plants, Momordica dioica is a dioecious climbing herb. It contains many phytoconstituents.One of it is a quaternary ammonium salt of benzyl-isoquinoline alkaloid called as berberine exhibiting a wide variety of pharmaceutical properties.In the current investigation, the activities of berberine for its anti-diabetic potential in the Momorica Dioica plant.The goal of the recent effort is to establish and validate an HPTLC method that is quick, accurate, exact, and specific for determining berberine from herbal extract of Momordica dioica. Methodology/Conclusions/significance: For a quick examination to determine the amount of berberine, a (HPTLC) process was created as well as validated.On HPTLC aluminium plate 60 F254,precoated with silica gel chromatographic separation was accomplished using the solvents: methanol ,ethyl acetate, toluene and formic acid (2:4:3:0.5). A wavelength of 348 nm was used for detection. Rf was found to be 0.44±5% for berberine. There was linearity for berberine in the 400ng/band concentration range.The LOD and LOQ was found to be0.0096ng/band and 0.0293ng/ band for Berberine.The average berberine recovery rate was (0.041).The method's precision, accuracy, linearity, robustness, and specificity were all authenticated in accordance with ICH guidelines. The discovered approach can be used to regularly analyse Berberine in herbal formulations for quality control.
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