New validated Ultra High Performance Liquid Chromatographic method for Estimation of Quercetin

Abstract

Background: A new Ultra performance liquid chromatographic analytical method was developed and validated for estimation of Quercetin in bulk powder. The reverse phase chromatographic elution using UPLC was carried out in gradient mode on C-18 column (Phenomenex Luna 5 µm, 150 mm X 4.6 mm) as stationary phase utilizing a mobile phase composed of Acetonitrile: 0.1 % formic acid buffer (50:50 v/v) with a flow rate of 1 ml/min and injection volume of 10µl.  The analysis was performed at temperature of 40⁰C and detection of eluent was carried out using photo diode array at 371 nm. The newly developed Ultra performance liquid chromatography method was validated in terms of linearity and range, system suitability, specificity, precision, sensitivity, robustness, ruggedness and accuracy as per ICH guidelines. Results: The retention time of quercetin was found to be at 3.9 min with total run time of analysis is 7 minutes. The linearity was observed between the concentration ranges from 0.5 to 16µg/ml with correlation coefficient 0.999. The precision assays values were found to be less than 2% for both the drugs. The Limit of Detection and Limit of Quantification were 0.47µg/ml and 1.44µg/ml for Quercetin. The mean percentage recovery values were found to be within the range of 90-110 %. Conclusion: The proposed method was found to be simple, specific, precise, sensitive, robust, rugged and accurate and can be used for routine quality control analysis of Quercetin.