In-Silico Anti-Diarrhoeal Evaluation of Lagu Gangathara Chooranam

Abstract

Background: In siddha system of medicine diarrhoea can be compared with kazhichal. Diarrhoea results from several factors like intestinal hyper-motility, malabsorbtion of water, inflammation of GIT or use of laxatives. In siddha literature, the preparation named lagu gangathara chooranam is indicated for kazhichal. Aim: The study aims to perform the in-silico computational studies of phytoconstituents of the siddha formulation lagu gangathara chooranam targeted against m3 muscarinic acetyl choline receptor for anti-diarrhoeal activity. Methods: The retrieved phytocomponents were subjected for docking calculations against target enzyme m3 muscarinic acetylcholine receptor. Results: The computational analysis retrived 11 bio-active compounds from herbal formulation. It includes limonene, α-humulene, cyperolone, lupeol, beta amyrin, betulinic acid, mangiferin, quercetin, caryophyllene and luteolin. These compounds significantly bind against the target  m3 muscarinic acetylcholine receptor. Hence these phytocompounds may produce significant anti-diarrheal activity by hindering the activity of m3 muscarinic acetylcholine receptor in the intestinal region which mediates the diarrhoea. Conclusion: Further clinical trials or experimental studies could be conducted to confirm the effectiveness of this siddha formulation lagu gangathara chooranam against diarrhoea.