Physico-chemical Analysis of immune modulatory formulation R9 vati

Abstract

Introduction: The concept of well-being has evolved to include health beyond disease absence. Rasayana refers to the nutritional essence reaching all body tissue components to nourish and replenish them. It enhances longevity, intelligence, health, youth, skin, voice, and motor and sensory strength. Rasayana herbs are used in decoctions or powder to achieve this, enhancing various dhatu (tissues) and promoting overall health. Ashwagandha, Pippali, Guduchi, and Yashtimadhu are herbs known for their anti-oxidant properties. Rasasindura, a rasashaudhi used to treat various illnesses, is a kupipakwa preparation with shuddha parada and shuddha gandhaka. These Rasaushadhi are effective in small doses and can enhance the efficacy of herbal drugs when added to formulations. Method: In this study a tablet (R9 vati) was prepared with and without rasasindura. The prepared samples were subjected to various physico chemical analysis. Observation and result: Increase in the value of Total ash, Alcohol and Water soluble extractive, uniformity of  weight, Hardness, and decrease in the value of   Loss on drying, Water soluble ash was observed in R9 vati prepared with rasasindura. HPTLC result shows 8 peaks in both samples of vati at Short UV, 6 peaks in both samples of vati at Long UV, and 4 peaks in both samples of vati at Post derivatization. Result of Microbial analysis shows absence of Escherichia coli, Staphylococcus aureus, Salmonella sp., Enterobacteriaceae and Pseudomonas aeruginosa in both the samples of vati.