Evaluation of In vitro Antioxidant and Antidiabetic activity of a Polyherbal formulation

Authors

  • Ramen Kalita
  • Bipul Nath
  • Dipankar Saha

DOI:

https://doi.org/10.47552/ijam.v14i4.4118

Keywords:

Diabetes, Herbal plant, Antioxidant, Antidiabetic, Polyherbal, Treatment

Abstract

Recent years have seen significant improvements in the identification, management, and study of metabolic illnesses, particularly diabetes. In addition to the present synthetic medicines, current research is focused on developing an alternate source of medication from natural resources for managing diabetes. Objective: To investigate the antioxidant and antidiabetic efficacy of a poly-herbal formulation (PHF) by in vitro models. Methods: The Polyherbal formulation was screened for the presence of phytochemical constituents. The total phenolic and flavonoids content of the formulation was also analysed.  The in vitro antioxidant and antidiabetic activity of the formulation (PHF) were calculated. Results: The Polyherbal formulation showed presence of phenols, flavonoids, steroids, and saponins. The IC50 value of PHF4 was found to be 42.70µg/ml in DPPH radical scavenging assay.  It has shown high levels of phenols (172.6±0.68 mg/g), flavonoids (137.3±0.91 mg/g) and had IC50 values of 422.50 µg/ml and 438.71 µg/ml for amylase and glucosidase, respectively. Conclusion: The results indicate that Polyherbal Formulation (PHF4) had significant antioxidant activity as well as a strong inhibitory effect on α -amylase and α -glucosidase. However, for further validation of the above findings, in vivo antidiabetic study of polyherbal formulation will be carried out using animal models.

Author Biographies

Ramen Kalita

Research Scholar, Royal School of Pharmacy, The Assam Royal Global University, Betkuchi, Guwahati, Assam. India. Department of Pharmacology, Pratiksha Institute of Pharmaceutical Sciences, Guwahati, Assam, India.

Bipul Nath

Department of Pharmaceutics, Royal School of Pharmacy, The Assam Royal Global University, Betkuchi, Guwahati, Assam. India.

Dipankar Saha

Department of Pharmacology, Girijananda Chowdhury University, Guwahati, Assam. India.

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Published

07-01-2024

How to Cite

Ramen Kalita, Nath, B. ., & Saha, D. (2024). Evaluation of In vitro Antioxidant and Antidiabetic activity of a Polyherbal formulation. International Journal of Ayurvedic Medicine, 14(4), 1033–1038. https://doi.org/10.47552/ijam.v14i4.4118

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Section

Research Articles