Phytochemical analysis, Antioxidant and Antimicrobial Screening of Shoot Extracts of Viola odorata linn. from Shikari Devi Wildlife Sanctuary, Himachal Pradesh, India

Authors

  • Sunny Dhiman
  • Dev Prakash Dahiya
  • Saurabh Sharma

DOI:

https://doi.org/10.47552/ijam.v15i1.4347

Keywords:

Chromatography, Stigmasterol, Flavonoids, Extraction, Phytochemicals, Screening

Abstract

The traditional healers made significant use of the Viola odorata of the Violaceae family for its many ethnopharmacological uses. The phytochemical composition and in vitro antioxidant activity of solvent extracts from Viola odorata shoots were studied, and the antibacterial potential of the optimized extract was also examined. The Soxhlet apparatus was used to extract phytochemicals from shoots in four different solvents, including n-hexane, butanol, methanol, and water.  High-performance liquid chromatography (HPLC) was used to analyze the stigmasterol profile of V. odorata shoots. The extracts were found to contain alkaloids, flavonoids, terpenoids, saponins, and phenolics, all of which were found in significant amounts in the methanolic extract. The methanolic extract also showed the highest radical scavenging activity (79.23 ± 0.44 %). TLC and HPLC profiling of methanolic extract revealed a considerable amount of stigmasterol in the shoots of Viola odorata. HPLC analysis of the methanolic shoot extract of the plant revealed the presence of 0.009 µg mL-1 stigmasterol. Optimized Methanolic extract showed respectable in-vitro antimicrobial activity against Bacillus subtilis (2.6 ± 0.41 mm zone of inhibition), E. coli (3.4 ± 0.71 mm zone of inhibition), and Candida albicans (3.5 ± 0.48 mm zone of inhibition). The outcomes demonstrated that the methanolic shoot extract of Viola odorata from Shikari Devi Wildlife Sanctuary, Himachal Pradesh, India, is a valuable herb for further screening and might be prepared for in-vivo animal studies.

Author Biographies

Sunny Dhiman

Research Scholar, Pharmaceutical Sciences, Department of Pharmacology, School of Pharmacy, Abhilashi University, Mandi. HP. India.

Dev Prakash Dahiya

Professor, Department of Pharmaceutical chemistry, School of Pharmacy, Abhilashi University, Mandi HP 175028, India.

Saurabh Sharma

Professor, Department of Pharmacology, Chandigarh college of Pharmacy, CGC Landran, Punjab 140307, India. 

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Published

01-04-2024

How to Cite

Dhiman, S., Dahiya, D. P. ., & Sharma, S. (2024). Phytochemical analysis, Antioxidant and Antimicrobial Screening of Shoot Extracts of Viola odorata linn. from Shikari Devi Wildlife Sanctuary, Himachal Pradesh, India. International Journal of Ayurvedic Medicine, 15(1), 212–218. https://doi.org/10.47552/ijam.v15i1.4347

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Section

Research Articles