Development of stability indicating RP-HPLC method for Quercetin estimation in Nanostructure liquid crystalline dispersion

Abstract

An attempt has been made to develop stability representing the reverse-phase High-Performance Liquid Chromatography (RP-HPLC) method for quantitative estimation of quercetin Nanostructure liquid crystalline dispersion. HPLC method was developed by C-18 column with the mobile phase comprising of 0.4% orthophosphoric acid [OPA]: Acetonitrile [ACN] (45:55, v/v), isocratically pumped with a rate of flow 1 mL/min and 255 nm detection wavelength. The method validation of HPLC parameters and degradation tests was analysed by International Conference on Harmonization (ICH) guidelines. The developed HPLC method was found to be specific, linear (r²>0.999) over the selected range of concentration 2-14μg/mL, precise, with the limit of detection (LOD) and limit of quantification (LOQ) as 0.021 and 0.064μg/mL respectively. The entrapment efficiency of quercetin for prepared Nanostructure Liquid Crystalline Dispersion (NLCD) was observed to be 94.55 %. The developed HPLC method was applied for the quantification of quercetin in NLCD. The quercetin peak was completely resolved without any disturbances from degrading products and additives. The developed HPLC method was found to be specific, sensitive, and adequate for the regular assay of marketed products, and NLCD nanoformulation containing quercetin.