Formulation and Analytical Development of Laxative Polyherbal Suspension

Abstract

The aim of study is to formulate and evaluate polyherbal suspension containing aqueous extracts of Cassia Fistula L, Terminalia Bellerica Gaertn, Terminalia Chebula Retzm, Operculina Turpethum L, and Baliospermum Montanum Muell for laxative use and develop a validated analytical method by HPLC. The excipients employed in the formulation were Sucrose, Xanthan gum, Tween 80, Sodium methylparaben, Sodium propylparaben and water. The suspension was evaluated for organoleptic properties, Sedimentation volume, Redispersibility, Flow rate, Viscosity, and pH. Optimized formulation was further evaluated to check its stability for 90 days in accelerated conditions. The analytical method was developed for the assay of Rhein in formulated polyherbal suspension and validated by HPLC (Waters instrument with Empower software). The results revealed that an increased concentration of suspending agent increased viscosity, thus reducing the sedimentation and improving the stability of suspension. The validation parameters results indicated that the developed method is simple, reproducible, accurate, robust, precise, and specific. Polyherbal suspension was successfully formulated using varying concentrations of suspending agent and wetting agent by employing a 3² factorial design. The validation results obtained for the assay of rhein in polyherbal formulation showed that the developed HPLC method was precise and accurate.