A novel sustainable and ecofriendly UV-Spectrophotometric method for the determination of Naringenin in bulk and vaginal niosomal nanoformulation

Authors

  • Anvitha Welda Sequeira
  • Kishori P Sutar
  • Utkarsh Shashtri
  • Sahil S Naik
  • Arun D Jadhav
  • Kallis X Dsouza

DOI:

https://doi.org/10.47552/ijam.v15i3.4960

Keywords:

PCOS, Naringenin, Niosomes, Forced degradation studies, Analytical method validation, Phosphate buffer pH 4.5

Abstract

Naringenin, a flavonoid found in several fruits like oranges, grapefruit, and bergamot, exhibits a wide range of biological effects. These include antidiabetic, antidepressant, antiatherogenic, antitumor, immunomodulatory, anti-inflammatory, hypolipidemic, antioxidant, peroxisome proliferator-activated receptors (PPARs) activation, hepatoprotective properties, memory enhancement and DNA protective properties. The aim of this current study is to develop a simple and precise UV spectrophotometric technique for measuring Naringenin Active Pharmaceutical Ingredient (API) in bulk and vaginal niosomal nanoformulation using PBS (Phosphate buffer solution) pH 4.5. The absorption peak of Naringenin was identified at 287 nm, and it adhered to Beer's law within the concentration range of 2 to 14 µg/ml. The calibration curve demonstrates a linear relationship between absorbance and concentration within the range of 2 to 14 µg/ml. The determined limit of detection and limit of quantification were found to be 0.587672µg/mL and 1.780824µg/mL, respectively. The validity of the method was assessed for repeatability, accuracy and precision. The results obtained indicated minimal intraday and interday variation. The excipients in the nanoformulation did not interfere the analysis. The developed analytical UV spectrophotometric method is simple, rapid and consistent, making it suitable for estimating the drug in both bulk and nanoformulation.

Author Biographies

Anvitha Welda Sequeira

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi-590010, Karnataka, India.

Kishori P Sutar

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi-590010, Karnataka, India.

Utkarsh Shashtri

Vasishta Pharmaceuticals Pvt Ltd., Antharapalya, Hoskerehalli, Bengaluru-560039, Karnataka, India.

Sahil S Naik

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi-590010, Karnataka, India.

Arun D Jadhav

Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi-590010, Karnataka, India.

Kallis X Dsouza

Department of Pharmaceutics, KLE College of Pharmacy, Hubballi, KLE Academy of Higher Education and Research, Vidya Nagar, Hubballi-580031, Karnataka, India.

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Published

06-10-2024

How to Cite

Sequeira, A. W. ., Sutar, K. P., Shashtri, U., Naik, S. S., Jadhav, A. D., & Dsouza, K. X. (2024). A novel sustainable and ecofriendly UV-Spectrophotometric method for the determination of Naringenin in bulk and vaginal niosomal nanoformulation. International Journal of Ayurvedic Medicine, 15(3), 641–649. https://doi.org/10.47552/ijam.v15i3.4960

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Section

Research Articles