Acute oral toxicity of herbo-mineral combination: Talisadi yoga in Wistar albino rats

Abstract

Talisadi yoga (TY) has been used as an herbal contraceptive. Before any product is released into the market, the toxicity study parameters are necessary to generate data that is safe for scientific use. An in vivo study was carried out on female Wistar albino rats at the Institute for Industrial Research and Toxicology (IIRT), Ghaziabad. The study aimed to evaluate the acute oral toxicity (AOT) study of TY by following the guidelines of OECD 425: AOT-Up and Down Procedure. Healthy female Wistar albino rats, of body weight 200±20 g, were selected for study and used stepwise according to the guidelines. The study was conducted with a single starting dose of 2000 mg/kg body weight. The test sample was administered orally at dose volume of 10 ml/kg to one female rat. The treated animal was closely observed for clinical signs of intoxication during the first four hours of TY administration. Thereafter, the animal was observed periodically at a 12-hour interval for 48 hours. No sign of toxicity or mortality was observed in the 48 h; hence, another two animals were dosed at the same dose level and observed for 48 h and finally for 14 days after the administration of the TY, respectively. Since three animals survived at the tested dose level (2000 mg/kg b. wt.), the TY tested for AOT, was found non-hazardous to Wistar albino rats. According to the Globally Harmonised System of Classification and Labelling of Chemicals (GHS), it comes under Category 4 (LD50 > 2000 mg/kg).