Evaluation of Analgesic and Anti-inflammatory activity study of Polyherbal formulation ACUPEN in experimental animal models

Abstract

Aim and Objective: Evaluation of Analgesic and Anti-Inflammatory activity study of polyherbal formulation ACUPEN in experimental animal models. Methodology: The acute toxicity test of the Polyherbal formulation ACUPEN was performed by OECD 423-guideline. Analgesic activity was evaluated using Eddy’s hot plate method & acetic acid-induced abdominal writhing test. The anti-inflammatory activity was measured using a digital vernier caliper with carrageenan-induced hind paw oedema. All drugs were given 1ml of 1% vehicle (CMC), Pentazocine 10mg/kg (i.p), Diclofenac 10mg/kg and Test drugs at doses 150, 300 and 600 mg/kg in 1% CMC (vehicle) were administered per orally in the experiment. Result and Discussion: Eddy’s Hot plate method results showed central analgesic activity through a long-lasting significant increase in response time from 30 minutes to 120 minutes in the Polyherbal formulation ACUPEN. In acetic acid-induced writhing ACUPEN exhibited dose-dependent peripheral analgesic activity. Amongst low, mid and high, the high dose (600mh/kg) showed significant (p<0.05) peripheral analgesic activity compared to standard drug. In the carrageenan-induced paw oedema method, the percentage inhibition of all the groups was compared at 1, 2, 3, 4, and 5 h, and it was found that high-dose ACUPEN (600mg/kg) showed highly significant P< 0.05 of % inhibition than the other groups. Conclusion: From the current study it has been concluded that the Polyherbal formulation ACUPEN was found to be safe for greater than 5000mg/kg body weight. The formulation exhibited both central and peripheral analgesic action in a dose-dependent manner in experimental models. The Polyherbal formulation has also exhibited anti-inflammatory activity too.