In Vitro Diffusion and Bioavailability Assessment of Gandharva Haritaki Churna: An Ayurvedic Herbal Formulation Study

Abstract

Medicated oils are used both externally and internally to treat a variety of illnesses. In Ayurvedic literature, Murchhana is a method used to improve the qualities of crude oil. In this study, Gandharva Haritaki Churna (Powder), a medicinal formulation, is made using two procedures: processed (Murchhita) Erand Taila (PET) and unprocessed (Amurchhita) Erand Taila (AET). The purpose of this study is to evaluate the in-vitro diffusion of Gandharva Haritaki Churna prepared using processed and crude castor oils. The in-vitro rate and permeability of a pharmacological dosage form are excellent indicators of the drug's absorption. The aim of this study is to assess the drug release of both powders utilizing in vitro absorbance methods using the Franz diffusion cell apparatus. The in-vitro absorbance was measured in a Franz diffusion cell equipment at pH 4, 7, and 9.2. The samples were collected and tested with a UV spectrophotometer to determine the absorbance of Churna (Powder) at various wavelengths. It was discovered that the buffer solution with a pH of 9.2 had the highest absorbance of all samples. This experimental study demonstrates that both formulations have good sustained absorption via the gastrointestinal tract. The results show that the rate of absorption was greater in PGHC compared to UGHC. This work contributes to the development of a new approach for evaluating intestine absorption and comparing formulations based on therapeutic efficacy and drug absorbance.