Mechanism of Arkeshwara rasa to combat hepatocellular carcinoma

Abstract

Background: Immunomodulatory treatments along with systemic and loco-regional methodology of treatment approach have reduced the incidence of Hepatocellular carcinoma (HCC) in recent observations. The phytomolecular applications have been seen to reverse the hepatic fibrosis aiding in regeneration of hepatocytes. The cytotoxicity of Arkeshwara rasa (AR) was investigated on HCC cell lines to observe the general signs of apoptosis. Methodology: The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay and Ethidium Bromide/Acridine orange Assay (EtBr/AO) were subjected on AR to know the cytotoxicity and the apoptotic results. Results: The IC50 of 37.14 μg/ml was exhibited by AR against Huh 7 cell lines and the (EtBr/ AO) Assay demonstrated the early and late apoptotic features in comparison with Untreated and Doxorubicin standard drug. Conclusion: AR on the basis of present evaluation against HCC cell lines and previous results on various other cell lines might be able to check the pathogenesis progression of HCC cells initiating the apoptotic features or reviving the vulnerable cell from further damage because of the phyto-constituents or mineral inclusions. These preliminary observations have to be again revalidated with reverse transcription, cDNA synthesis, western blotting, cell cycle analysis, etc.