In-vitro antioxidant, anticancer, wound healing activity profile on aerial parts of Phyllanthus reticulatus poir.

Abstract

Background: Antioxidants and anticancer herbs play a major role in preventing and curing current life-threatening disorders due to their cell damage repair properties.  Phyllanthus reticulatus Poir. is a shrub, growing commonly in tropical areas, aerial parts (PRAP) of which are used for malignancy, wound healing, and as a rejuvenator. Materials and Methods: PRAP was collected, aqueous extract of the Phyllanthus reticulatus Poir. leaves (PRL) was screened for antioxidant (free radical scavenging activity and reducing power assay), anticancer activity (MTT assay), and wound healing activity on HEK 293 cell line as per standard protocol. Results: Both free radical scavenging activity and reducing power assay have shown positive results, compared to Vitamin C, in higher concentrations. In the MTT assay, as the concentration of test drug extract increased from lower to higher (1 to 5000 µg/mL), % viability was gradually decreased up to 5.3 %, thus showing better cytotoxic activity. This study proved the efficacy of PRL as an antioxidant, anticancer, and wound healing activity agent. Conclusion: Drugs possessing antioxidant, wound healing, and anticancer activities is the need of the hour to prevent lethal diseases like malignancy. Aqueous extract of aerial parts P. reticulatus has shown promising results in the pre-clinical model, which can be taken for further study.