Evaluation of Anti-bacterial activity of Charakokta Vishaghna Gana tablet against Cutibacterium acnes: An In-vitro study

Abstract

The present research study aims to assess and authenticate classical recipes with scientific evidence. To evaluate the in vitro anti-bacterial potential of Vishaghna Gana tablet and its constituents against Cutibacterium acnes, a causative organism of  Acne vulgaris. This study also assessed the morphological and physicochemical parameters of the Vishaghna Gana tablet. The results of morphological and physicochemical tests of the tablets were hardness, weight variation, friability, thickness, disintegration, ash value, extractive value and ph, as per CCRAS pharmacopeial standards. The formulated tablet showed a rounded shape with yellowish-brown color and typical odour and taste. The antibacterial screening was carried out by disc diffusion and punch well techniques on Brucella Blood Agar under anaerobic conditions and compared with azithromycin as a standard control. Tests were done in triplicate for reliability. In an in-vitro antibacterial assay, the zone of inhibition for Sireesha and Kataka showed the highest activity with inhibition zones >18 mm. In contrast, Haridra, Manjistha, Chandana and Slesmataka were moderately active with inhibition zones between 13–18 mm. The methanolic extracts of the  Vishaghna Gana tablet showed remarkable antibacterial activity against C.acnes. These findings validate Sireesha as an Agrya aushadha (best medicine) for Visha(poison) as quoted in Ayurveda classics and corroborate Kataka's folk usage in water purification (Ambu prasadina). The results underscore the necessity of optimising extraction techniques and concentrations to improve antibacterial activity.