A Research on Comparative Methods of Isolation, Evaluation and Identification of Clitoria ternatea Plant

Abstract

Clitoria ternatea (Butterfly pea) is a traditional medicinal plant known for its therapeutic properties, including antimicrobial, antioxidant, and anti-inflammatory activities. This study aimed to evaluate its phytochemical composition, physicochemical characteristics, and extraction efficiency using different techniques to support its pharmacological potential. Ethanolic extracts of Clitoria ternatea flowers were prepared using maceration, Soxhlet extraction, and ultrasonic-assisted extraction. Qualitative phytochemical screening was conducted to identify secondary metabolites, while physicochemical parameters such as total ash, water-soluble ash, loss on drying, and alcohol- and water-soluble extractives were assessed. The Soxhlet method yielded the highest extractive value (89%), followed by maceration (62%) and ultrasonic extraction (57%). Phytochemical screening confirmed the presence of key bioactive compounds, including flavonoids, alkaloids, saponins, tannins, steroids, and cardiac glycosides. Physicochemical evaluations were within acceptable limits, supporting extract quality and reproducibility. This study validates the traditional use of Clitoria ternatea and demonstrates its potential for further development as a phytopharmaceutical agent. Soxhlet extraction is recommended for optimal recovery of bioactive constituents. Further research is warranted to isolate specific compounds and explore their therapeutic applications through pharmacodynamic and clinical studies.