Evaluation of Rasanjanadi Churna in Acetic acid induced Ulcerative Colitis in Rats

Abstract

Introduction: Ulcerative Colitis is an idiopathic form of acute and chronic ulcero-inflammatory colitis affecting chiefly the mucosa and submucosa of the rectum and descending colon. Drug resistance, drug dependency and side effects of those drugs are seen in long term treatment according to modern medicine. The signs and symptoms of Ulcerative Colitis show much resemblance with the symptoms of Raktatisaar. Therefore, an Ayurvedic herbal formulation namely Rasanjanadi Churna mentioned in Yogratnakar Atisaar Adhyaya Raktatisaar Adhikaar is chosen for study in Ulcerative Colitis. Objective- To evaluate the efficacy of Rasanjanadi Churna in Acetic acid induced Ulcerative Colitis in Albino Wistar Rats. Materials and Method- 24 Rats were randomly selected into 4 groups namely Disease Control, Standard, Test and Vehicle. All groups were given drugs from 1 to 7 days and colitis was induced on Day 4 by Intrarectal administration of 500 µL acetic acid (4%). On Day 8, all animals were sacrificed and parameters were studied. Observation and Result- Assessment criteria were Body Weight, Clinical Sign and Symptom, Assessment of Stool, Macroscopic assessment of Colon, Haematological parameter, Biomarker oxidative Assay (Catalase, Superoxide dismutase and Malonaldehyde), Serum IL-6, Serum IgA parameter, Microscopic assessment of Colon, Colon weight to length ratio. Conclusion- The findings of the Pre- Clinical study indicate that Rasanjanadi Churna showed positive results against the damaging effect of acetic acid induced colitis in Wistar rats. Rasanjanadi Churna showed equivalent results in all the parameters when compared with Standard drug Sulphasalazine.